The Human TFR (CD71) Transferrin Receptor CLIA Kit is specially designed for the accurate and precise measurement of transferrin receptor (CD71) levels in human serum, plasma, and cell culture supernatants. This innovative kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.The transferrin receptor is a critical protein involved in iron metabolism, playing a key role in the uptake of iron from transferrin into cells. Dysregulation of transferrin receptor expression has been linked to various conditions such as iron deficiency anemia, cancer, and neurodegenerative disorders, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
With its advanced technology and superior performance, the Human TFR (CD71) Transferrin Receptor CLIA Kit is a valuable tool for researchers looking to uncover the role of transferrin receptor in health and disease. Order yours today and take your research to the next level.
Product Name:
Human TFR/CD71 (Transferrin Receptor) CLIA Kit
SKU:
HUES01213
Target:
Human TFR/CD71 (Transferrin Receptor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TFR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TFR1. You can calculate the concentration of Human TFR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
97-113
86-102
Average (%)
108
104
93
1:4
Range (%)
92-104
90-107
101-116
Average (%)
99
97
108
1:8
Range (%)
89-104
90-104
101-117
Average (%)
97
96
109
1:16
Range (%)
99-113
98-109
99-115
Average (%)
105
104
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
104
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
102-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.19
7.31
1.1
2.31
7.73
Standard deviation
0.12
0.18
0.65
0.1
0.17
0.49
C V (%)
11.32
8.22
8.89
9.09
7.36
6.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TFR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFR1 in samples. No significant cross-reactivity or interference between Human TFR1 and analogues was observed.
