Human TFPI2 (Tissue Factor Pathway Inhibitor 2) CLIA Kit
The Human TFPI2 (Tissue Factor Pathway Inhibitor 2) CLIA Kit is a powerful tool for the precise measurement of TFPI2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TFPI2 is a key regulator of the tissue factor pathway, playing a critical role in controlling blood coagulation and preventing excessive blood clot formation. Dysregulation of TFPI2 has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions.
The ability to accurately quantify TFPI2 levels can provide valuable insights into disease pathogenesis and aid in the development of targeted therapeutic interventions.With its advanced technology and reliable performance, the Human TFPI2 CLIA Kit is an essential tool for researchers studying the role of TFPI2 in health and disease. Don't miss out on the opportunity to enhance your research with this cutting-edge assay. Visit assaygenie.com/human-tfpi2-tissue-factor-pathway-inhibitor-2-clia-kit-hues01220/ to learn more and place your order today.
Product Name:
Human TFPI2 (Tissue Factor Pathway Inhibitor 2) CLIA Kit
SKU:
HUES01220
Target:
Human TFPI2 (Tissue Factor Pathway Inhibitor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TFPI2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFPI2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFPI2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TFPI2. You can calculate the concentration of Human TFPI2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
95-111
89-103
Average (%)
91
102
96
1:4
Range (%)
90-105
95-109
95-112
Average (%)
96
102
102
1:8
Range (%)
97-115
99-113
96-114
Average (%)
105
107
104
1:16
Range (%)
99-113
96-108
98-115
Average (%)
106
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.33
93.86
349.9
46.43
88.51
345.05
Standard deviation
4.9
8.69
24.07
3.82
7.93
31.88
C V (%)
10.35
9.26
6.88
8.23
8.96
9.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TFPI2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFPI2 in samples. No significant cross-reactivity or interference between Human TFPI2 and analogues was observed.
