Human TFF3 (Trefoil Factor 3, Intestinal ) CLIA Kit
The Human TFF3 (Trefoil Factor 3) Intestinal CLIA Kit is a reliable tool for the accurate detection of TFF3 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and trustworthy results, making it valuable for various research applications.TFF3 is a vital protein involved in maintaining intestinal mucosal integrity and promoting mucosal healing.
Dysfunction of TFF3 has been associated with inflammatory bowel diseases, gastric ulcers, and other gastrointestinal disorders, making it a valuable biomarker for studying and developing therapies for such conditions.Overall, the Human TFF3 Intestinal CLIA Kit is a valuable tool for researchers and clinicians alike, offering precise and accurate measurements of TFF3 levels for a deeper understanding of intestinal health and disease.
Product Name:
Human TFF3 (Trefoil Factor 3, Intestinal ) CLIA Kit
SKU:
HUES00651
Target:
Human TFF3 (Trefoil Factor 3, Intestinal )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TFF3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFF3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TFF3. You can calculate the concentration of Human TFF3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
95-107
103-117
Average (%)
96
101
109
1:4
Range (%)
91-108
93-104
95-108
Average (%)
99
98
100
1:8
Range (%)
90-103
93-109
97-112
Average (%)
98
100
104
1:16
Range (%)
96-112
88-104
92-107
Average (%)
104
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.19
148.02
364.57
52.37
140.58
373.33
Standard deviation
5.46
15.66
37.59
5.22
13.59
24.08
C V (%)
11.33
10.58
10.31
9.97
9.67
6.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TFF3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFF3 in samples. No significant cross-reactivity or interference between Human TFF3 and analogues was observed.
