Human TERT (Telomerase Reverse Transcriptase) ELISA Kit
The Human TERT (Telomerase Reverse Transcriptase) ELISA Kit is specifically designed for the precise detection of TERT levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results, perfect for various research purposes.TERT is a key enzyme involved in maintaining telomere length, crucial for cell division and genomic stability.
Its dysregulation has been linked to aging, cancer, and other diseases, highlighting its importance as a biomarker for studying these conditions and exploring potential treatments.Overall, the Human TERT ELISA Kit from Assay Genie is an invaluable tool for researchers looking to uncover the role of TERT in health and disease, providing accurate and reliable data for in-depth analyses.
Product Name:
Human TERT (Telomerase Reverse Transcriptase) ELISA Kit
SKU:
HUES01857
Target:
Human TERT (Telomerase Reverse Transcriptase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TERT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TERT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TERT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TERT. You can calculate the concentration of Human TERT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
84-96
89-101
Average (%)
105
91
94
1:4
Range (%)
92-106
82-95
88-99
Average (%)
99
88
93
1:8
Range (%)
88-103
85-96
85-97
Average (%)
95
91
92
1:16
Range (%)
93-105
85-97
84-94
Average (%)
99
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
95-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.58
4.0
0.44
1.58
4.34
Standard deviation
0.03
0.07
0.19
0.02
0.08
0.15
C V (%)
6.25
4.43
4.75
4.55
5.06
3.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TERT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TERT in samples. No significant cross-reactivity or interference between Human TERT and analogues was observed.
