Human TDP43 (Tar DNA Binding Protein 43KDa ) ELISA Kit
The Human TDP43 (Tar DNA Binding Protein 43kDa) ELISA Kit is specially designed for the precise measurement of TDP43 levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.TDP43 is a vital protein involved in RNA processing and regulation, with implications in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD).
By detecting TDP43 levels, researchers can gain insights into the pathogenesis of these disorders and explore potential therapeutic interventions.Overall, the Human TDP43 ELISA Kit offers researchers a reliable and efficient method for investigating the role of TDP43 in disease pathogenesis, paving the way for new advancements in diagnosis and treatment strategies.
Product Name:
Human TDP43 (Tar DNA Binding Protein 43KDa ) ELISA Kit
SKU:
HUES02066
Target:
Human TDP43 (Tar DNA Binding Protein 43KDa )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TDP43. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TDP43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TDP43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TDP43. You can calculate the concentration of Human TDP43 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
100-113
92-105
Average (%)
92
106
97
1:4
Range (%)
89-101
87-100
83-95
Average (%)
94
93
87
1:8
Range (%)
90-101
84-97
86-97
Average (%)
96
91
91
1:16
Range (%)
89-105
83-93
88-104
Average (%)
96
87
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
90-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
3.03
7.97
1.02
3.04
7.39
Standard deviation
0.06
0.16
0.34
0.07
0.13
0.4
C V (%)
5.56
5.28
4.27
6.86
4.28
5.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TDP43 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TDP43 in samples. No significant cross-reactivity or interference between Human TDP43 and analogues was observed.
