Human TCF4 Recombinant Protein (RPPB4927)
- SKU:
- RPPB4927
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- TCF4
- Synonyms:
- Transcription factor 4
- TCF-4
- Class B basic helix-loop-helix protein 19
- Immunoglobulin transcription factor 2
- Source:
- Escherichia Coli
- Uniprot:
- P15884
Description
| Product Name: | Human TCF4 Recombinant Protein |
| Product Code: | RPPB4927 |
| Size: | 10µg |
| Species: | Human |
| Target: | TCF4 |
| Synonyms: | Transcription factor 4, TCF-4, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, TCF4, bHLHb19, E2-2, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TCF4 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMKFKQCR CSDTGLCCLD HEGKAEVYAP SASTADYNRD SPGYPSSKPA TSTFPSSFFM QDGHHSSDPW SSSSGMNQPG YAGMLGNSSH IPQSSSYCSL HPHERLSYPS HSSADINSSL PPMSTFHRSG TNHYSTSSCT PPANGTDSIM ANRGSGAAGS SQTGDALGKA LASIYSPDHT NNSFSSNPST PVGSPPSLSA GTAVWSRNGG QASSSPNYEG PLHSLQSRIE DRLERLDDAI HVLRNHAVGP STAMPGGHGD MHGIIGPSHN GAMGGLGSGY GTGLLSANRH SLMVGTHRED GVALRGSHSL LPNQVPVPQL PVQSATSPDL NPPQDPYRGM PPGLQGQSVS SGSSEIKSDD EGDENLQDTK SSEDKKLDDD KKDIKSITSN NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP LSLAGPHPGM GDASNHMGQM |
Transcription Factor 4 (TCF4) acts as a transcription factor and takes part in the initiation of neuronal differentiation. TCF4 recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif primary identified in immunoglobulin enhancers. TCF4 is mainly expressed in pre-B-cells, even though it is present in other tissues as well.
TCF4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 530 amino acids (1-507) and having a molecular mass of 56.6 kDa.TCF4 is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TCF4: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2. Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 18q21.1 Cellular Component: transcription factor complex; nucleus Molecular Function:protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; protein-DNA complex assembly Disease: Corneal Dystrophy, Fuchs Endothelial, 3; Pitt-hopkins Syndrome |
| NCBI Summary: | This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P15884 |
| NCBI GenInfo Identifier: | 3915747 |
| NCBI Gene ID: | 6925 |
| NCBI Accession: | P15884.3 |
| UniProt Secondary Accession: | P15884,B3KT62, B3KUC0, B4DT37, B4DUG3, B7Z5M6, B7Z6Y1 G0LNT9, G0LNU0, G0LNU1, G0LNU2, G0LNU4, |
| UniProt Related Accession: | P15884 |
| Molecular Weight: | 48,098 Da |
| NCBI Full Name: | Transcription factor 4 |
| NCBI Synonym Full Names: | transcription factor 4 |
| NCBI Official Symbol: | TCF4 |
| NCBI Official Synonym Symbols: | E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19 |
| NCBI Protein Information: | transcription factor 4; SL3-3 enhancer factor 2; transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform R; immunoglobulin transcription factor 2; class B basic helix-loop-helix protein 19 |
| UniProt Protein Name: | Transcription factor 4 |
| UniProt Synonym Protein Names: | Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | TCF4 |
| UniProt Entry Name: | ITF2_HUMAN |
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