Human TBP2 (Thioredoxin Binding Protein 2) ELISA Kit
The Human TBP2 (Thioredoxin Binding Protein 2) ELISA Kit is specifically designed for the quantitative measurement of TBP2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.TBP2, also known as thioredoxin binding protein 2, plays a crucial role in regulating cellular redox balance and modulating cellular responses to oxidative stress. Dysregulation of TBP2 has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and user-friendly format, the Human TBP2 ELISA Kit from Assay Genie provides researchers with a reliable tool for investigating the role of TBP2 in disease pathogenesis and therapeutic development. Order yours today and unlock new possibilities in your research endeavors.
Product Name:
Human TBP2 (Thioredoxin Binding Protein 2) ELISA Kit
SKU:
HUES02717
Target:
Human TBP2 (Thioredoxin Binding Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TBP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TBP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TBP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TBP2. You can calculate the concentration of Human TBP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
91-105
96-109
Average (%)
104
98
103
1:4
Range (%)
91-103
79-93
86-102
Average (%)
97
85
93
1:8
Range (%)
87-100
84-96
86-99
Average (%)
94
91
93
1:16
Range (%)
92-108
86-97
87-101
Average (%)
100
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-106
97
EDTA plasma (n=5)
94-111
101
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.15
4.2
0.44
1.13
3.9
Standard deviation
0.03
0.06
0.17
0.03
0.06
0.16
C V (%)
6.52
5.22
4.05
6.82
5.31
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TBP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed.
