Human TAT (Thrombin/Antithrombin Complex) ELISA Kit
The Human TAT (Thrombin-Antithrombin Complex) ELISA Kit is specifically designed for the accurate measurement of thrombin-antithrombin complex levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Thrombin-antithrombin complex is a key indicator of blood coagulation and plays a critical role in the regulation of hemostasis.
Abnormal levels of this complex are associated with various thrombotic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human TAT ELISA Kit from Assay Genie provides researchers with a reliable tool for investigating thrombin-antithrombin complex levels and their implications in a range of physiological and pathological processes.
Product Name:
Human TAT (Thrombin/Antithrombin Complex) ELISA Kit
SKU:
HUES02758
Target:
Human TAT (Thrombin/Antithrombin Complex)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TAT. You can calculate the concentration of Human TAT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
99-114
98-115
Average (%)
103
106
105
1:4
Range (%)
89-102
88-100
82-96
Average (%)
95
93
88
1:8
Range (%)
87-101
82-95
82-97
Average (%)
92
87
89
1:16
Range (%)
87-99
81-91
84-99
Average (%)
93
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
91
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
90-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.18
142.33
347.18
59.09
153.05
370.25
Standard deviation
3.74
6.43
13.89
3.85
8.23
13.44
C V (%)
6.9
4.52
4.0
6.52
5.38
3.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TAT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TAT in samples. No significant cross-reactivity or interference between Human TAT and analogues was observed.
