Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit
The Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit is specifically designed for the accurate measurement of TARC levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.TARC is a chemokine that plays a key role in immune responses and inflammation regulation. It is involved in attracting specific immune cells to sites of inflammation, making it a valuable biomarker for studying inflammatory conditions, allergies, and autoimmune diseases.
With the Human TARC ELISA Kit, researchers can effectively measure TARC levels in biological samples, providing insights into disease mechanisms and potential therapeutic targets. Trust in the reliability and precision of this kit for your research needs.
Product Name:
Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit
SKU:
HUES01313
Target:
Human TARC (Thymus Activation Regulated Chemokine)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TARC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TARC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TARC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TARC. You can calculate the concentration of Human TARC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
93-107
88-99
Average (%)
101
99
94
1:4
Range (%)
85-98
80-94
83-94
Average (%)
92
86
89
1:8
Range (%)
91-107
82-96
86-98
Average (%)
98
88
91
1:16
Range (%)
90-107
83-92
90-104
Average (%)
98
88
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.26
217.44
679.06
102.11
209.1
668.22
Standard deviation
6.47
12.24
21.12
6.98
10.69
22.25
C V (%)
6.79
5.63
3.11
6.84
5.11
3.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TARC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TARC in samples. No significant cross-reactivity or interference between Human TARC and analogues was observed.
