Human TACE (TNF alpha Converting Enzyme) ELISA Kit
The Human TACE (TNF-alpha Converting Enzyme) ELISA Kit is specially designed for the precise and sensitive detection of TACE levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high accuracy and reliability, ensuring consistent and reproducible results, making it perfect for a variety of research purposes.TACE, also known as ADAM17, is a key enzyme involved in the shedding of cell surface molecules, including pro-inflammatory cytokines like TNF-alpha.
Dysregulation of TACE activity has been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.This ELISA kit provides researchers with a powerful tool to study the role of TACE in inflammatory pathways, paving the way for new insights and discoveries in the field of immunology and inflammation-related diseases. Get your Human TACE ELISA kit now and unlock the potential of your research.
Product Name:
Human TACE (TNF alpha Converting Enzyme) ELISA Kit
SKU:
HUES03182
Target:
Human TACE (TNF alpha Converting Enzyme)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TACE/ADAM17. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TACE/ADAM17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TACE/ADAM17, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TACE/ADAM17. You can calculate the concentration of Human TACE/ADAM17 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-105
90-102
95-108
Average (%)
96
97
100
1:4
Range (%)
89-101
84-96
82-97
Average (%)
96
90
88
1:8
Range (%)
89-103
85-97
84-98
Average (%)
94
90
90
1:16
Range (%)
87-103
84-99
86-101
Average (%)
94
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.68
405.03
1437.11
219.74
410.66
1572.95
Standard deviation
11.01
18.63
45.41
15.27
22.59
81.79
C V (%)
5.43
4.6
3.16
6.95
5.5
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TACE/ADAM17 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TACE/ADAM17 in samples. No significant cross-reactivity or interference between Human TACE/ADAM17 and analogues was observed.
