The Human SYNPO (Synaptopodin) ELISA Kit is specifically designed for the precise measurement of synaptopodin levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Synaptopodin is a key protein involved in the regulation of actin dynamics in dendritic spines, contributing to synaptic plasticity and neuronal function.
Dysregulation of synaptopodin has been implicated in various neurological disorders, including Alzheimer's disease and schizophrenia, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions.Overall, the Human SYNPO (Synaptopodin) ELISA Kit provides researchers with a valuable tool for investigating the role of synaptopodin in neuronal function and its potential implications in neurodegenerative diseases.
Product Name:
Human SYNPO (Synaptopodin) ELISA Kit
SKU:
HUES02943
Target:
Human SYNPO (Synaptopodin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SYNPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SYNPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SYNPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SYNPO. You can calculate the concentration of Human SYNPO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-95
91-108
85-97
Average (%)
90
98
91
1:4
Range (%)
88-100
86-100
87-102
Average (%)
95
91
94
1:8
Range (%)
89-103
83-97
83-93
Average (%)
96
90
87
1:16
Range (%)
87-104
84-94
84-97
Average (%)
95
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-110
100
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.51
4.56
0.47
1.58
4.59
Standard deviation
0.02
0.06
0.18
0.02
0.07
0.17
C V (%)
4.35
3.97
3.95
4.26
4.43
3.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SYNPO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed.
