Human SUB1 Recombinant Protein (RPPB4872)

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SKU:
RPPB4872
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
SUB1
Synonyms:
p14
Synonyms:
P15
Synonyms:
PC4
Synonyms:
Positive cofactor 4
Source:
Escherichia Coli
Uniprot:
P53999
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Description

Product Name:Human SUB1 Recombinant Protein
Product Code:RPPB4872
Size:10µg
Species:Human
Target:SUB1
Synonyms:p14, P15, PC4, Positive cofactor 4, SUB1 homolog, RPO2TC1, Activated RNA polymerase II transcriptional coactivator p15, MGC102747.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:SUB1 Human solution containing 20mM Tris-HCl pH-8, 0.2M NaCl, 5mM DTT & 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPKSKELVSS SSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK VLIDIREYWM DPEGEMKPGR KGISLNPEQW SQLKEQISDI DDAVRKL

SUB1 is a coactivator that functions together with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP)-associated factors (TAFs) to directly bind to double stranded DNA. SUB1 induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components.

SUB1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids (1-127 a.a.) and having a molecular mass of 16.5 kDa. SUB1 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

UniProt Protein Function:PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: transcription factor complex; nucleolus; nucleus

Molecular Function:protein binding; transcription coactivator activity; single-stranded DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

UniProt Code:P53999
NCBI GenInfo Identifier:1709514
NCBI Gene ID:10923
NCBI Accession:P53999.3
UniProt Secondary Accession:P53999,Q96L29,
UniProt Related Accession:P53999
Molecular Weight:127
NCBI Full Name:Activated RNA polymerase II transcriptional coactivator p15
NCBI Synonym Full Names:SUB1 homolog (S. cerevisiae)
NCBI Official Symbol:SUB1  
NCBI Official Synonym Symbols:P15; PC4; p14  
NCBI Protein Information:activated RNA polymerase II transcriptional coactivator p15; positive cofactor 4; activated RNA polymerase II transcription cofactor 4
UniProt Protein Name:Activated RNA polymerase II transcriptional coactivator p15
UniProt Synonym Protein Names:Positive cofactor 4; PC4; SUB1 homolog; p14
Protein Family:Subtilisin
UniProt Gene Name:SUB1  
UniProt Entry Name:TCP4_HUMAN
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