Serine/threonine-protein kinase 16, also known as myristoylated and palmitoylated serine/threonine-protein kinase, Protein kinase PKL12, TGF-beta-stimulated factor 1, TSF-1, MPSK1 and STK16, is a membrane protein which is ubiquitously expressed at very low levels. STK16 / MPSK1 belongs to the protein kinase superfamily and Ser/Thr protein kinase family. It contains one protein kinase domain. Transforming growth factor-beta (TGF-beta) shows a variety of biological activities in various organs or cells. Some factors such as Smads (Sma and Mad proteins) and TGF-beta activating kinase 1 have been characterized as signalling molecules downstream of TGF-beta. Several TGF-beta response elements have been identified such as cAMP response element, Smad binding element, and recognition sites for activating protein-1 and stimulating protein-1 in various gene promoters. STK16 / MPSK1 is an unique factor with two biological functions, transcriptional regulation and protein phosphorylation, that may be involved in TGF-beta signals. STK16 / MPSK1 is a protein kinase that act on both serine and threonine residues. STK16 / MPSK1 possessed DNA-binding ability and activated the TGF-beta responsive CNP promoter or vascular endothelial growth factor gene promoter which possesses a sequence element analogous to the TGF-beta responsive GC-rich element of the CNP promoter. STK16 / MPSK1 did not directly activate a Smads-dependent promoter from plasminogen activator inhibitor 1 gene, but it showed enhancement in co-operation with Smad3 and Smad4. STK16 / MPSK1 mRNA as well as its protein level were stimulated by TGF-beta treatment.
Product Name:
Human STK16/PKL12/MPSK Recombinant Protein (RPES0943)
Product Code:
RPES0943
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
KRCT,MPSK,PKL12,TSF1
Accession:
AAH02618.1
Sequence:
Met 1-Ile 305
Fusion tag:
N-His & NusA
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the native human STK16 (AAH02618.1) (Met 1-Ile 305) was fused with the polyhistidine-tagged NusA tag at the N-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
92 kDa
AP Mol Mass:
105 kDa
Formulation:
Supplied as sterile PBS, pH 7.4
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
