Serine / threonine-protein kinase 10, also known as Lymphocyte-oriented kinase, STK10 and LOK, which belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and STE20 subfamily. Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells. The Ste20 family of serine/threonine kinases plays an important role in numerous cellular functions such as growth, apoptosis, and morphogenesis. STK10 is similar to several known polo-like kinase kinases. It can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. STK10 can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. Stk10 can associate with Plk1 in cells and furthermore can phosphorylate Plk1. It can also act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
Product Name:
Human STK10/LOK Recombinant Protein (RPES2640)
Product Code:
RPES2640
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
LOK,PRO2729
Accession:
NP_005981.3
Sequence:
Arg 18-Glu 317
Fusion tag:
N-His
Activity:
The specific activity was determined to be 1353 nmol/min/mg using synthetic AXLtide peptide (KKSRGDYMTMQIG) as substrate.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human STK10 (NP_005981.3) (Arg 18-Glu 317) was expressed, with a polyhistide tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
36 kDa
AP Mol Mass:
40 kDa
Formulation:
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
