Human sTfR1 (Soluble Transferrin Receptor1) CLIA Kit
Based on the information from the provided URL, the Human STFR1 (Soluble Transferrin Receptor1) CLIA Kit is a reliable assay kit designed for the precise measurement of soluble transferrin receptor1 levels in human samples. This kit offers high sensitivity and specificity, ensuring accurate and consistent results that are suitable for a variety of research purposes.Soluble transferrin receptor1 is a key protein involved in iron metabolism, serving as a marker for iron deficiency and anemia. Monitoring levels of soluble transferrin receptor1 can provide valuable insights into iron status and associated health conditions, making this kit essential for studies related to iron metabolism and related disorders.
Overall, the Human STFR1 CLIA Kit is a valuable tool for researchers and clinicians seeking to investigate and monitor soluble transferrin receptor1 levels in human samples, offering reliable results that can contribute to advancements in the understanding and treatment of iron-related disorders.
Product Name:
Human sTfR1 (Soluble Transferrin Receptor1) CLIA Kit
SKU:
HUES00928
Target:
Human sTfR1 (Soluble Transferrin Receptor1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 pmol/mL
Detection range:
0.63-40 pmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human sTfR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sTfR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sTfR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human sTfR1. You can calculate the concentration of Human sTfR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
87-99
103-121
Average (%)
92
93
110
1:4
Range (%)
84-100
95-111
92-104
Average (%)
91
103
98
1:8
Range (%)
99-115
86-99
100-114
Average (%)
105
93
107
1:16
Range (%)
100-118
85-99
96-111
Average (%)
108
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
86-102
93
Cell culture media (n=5)
102-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.2
4.74
17.16
2.15
5.13
17.58
Standard deviation
0.25
0.38
1.93
0.22
0.36
1.37
C V (%)
11.36
8.02
11.25
10.23
7.02
7.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human sTfR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sTfR1 in samples. No significant cross-reactivity or interference between Human sTfR1 and analogues was observed.
