Human STAT4 (Signal Transducer and Activator of Transcription 4) CLIA Kit
The Human STAT4 (Signal Transducer and Activator of Transcription 4) CLIA Kit is a powerful tool designed for the quantitative measurement of STAT4 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.STAT4 is an important transcription factor that plays a key role in regulating immune responses and inflammation. Abnormal STAT4 expression has been linked to autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus, making it a valuable biomarker for studying these conditions and developing potential therapies.
With its advanced technology and precision, the Human STAT4 CLIA Kit is a must-have for researchers looking to investigate the role of STAT4 in autoimmune diseases and other inflammatory disorders. Trust in this kit to provide you with the accurate data you need to advance your research efforts.
Product Name:
Human STAT4 (Signal Transducer and Activator of Transcription 4) CLIA Kit
SKU:
HUES01132
Target:
Human STAT4 (Signal Transducer and Activator of Transcription 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human STAT4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human STAT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human STAT4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human STAT4. You can calculate the concentration of Human STAT4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
99-113
92-103
Average (%)
100
106
98
1:4
Range (%)
100-113
99-113
100-115
Average (%)
107
104
106
1:8
Range (%)
97-108
88-98
94-109
Average (%)
102
93
102
1:16
Range (%)
96-114
87-100
86-100
Average (%)
104
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
103
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.61
493.57
1945.38
180.49
498.78
1786.91
Standard deviation
22.63
56.17
204.65
18.25
38.01
186.55
C V (%)
12.06
11.38
10.52
10.11
7.62
10.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human STAT4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human STAT4 in samples. No significant cross-reactivity or interference between Human STAT4 and analogues was observed.
