The Human ST2 (Syntenin-2) CLIA Kit is specifically designed for the precise quantification of ST2 (Syntenin-2) levels in human samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.ST2 (Syntenin-2) is a key protein involved in several physiological processes, including cell adhesion, migration, and signaling.
Dysregulation of ST2 has been linked to various diseases, including cancer, inflammatory disorders, and cardiovascular diseases, making it a valuable biomarker for understanding these conditions and potentially developing targeted therapies.Overall, the Human ST2 (Syntenin-2) CLIA Kit is a powerful tool for researchers seeking to investigate the role of ST2 in health and disease, providing reliable and precise measurements for their studies.
Product Name:
Human ST2 (Syntenin 2) CLIA Kit
SKU:
HUES00761
Target:
Human ST2 (Syntenin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ST2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ST2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ST2. You can calculate the concentration of Human ST2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
97-114
96-107
Average (%)
108
105
101
1:4
Range (%)
102-115
85-96
102-119
Average (%)
109
91
108
1:8
Range (%)
99-113
99-117
96-109
Average (%)
107
107
101
1:16
Range (%)
93-108
91-104
98-111
Average (%)
99
99
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
90
EDTA plasma (n=5)
88-103
96
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.1
218.33
952.29
84.6
199.19
1043.67
Standard deviation
10.13
19.06
106.66
10.08
18.37
109.06
C V (%)
11.12
8.73
11.2
11.91
9.22
10.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ST2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ST2 in samples. No significant cross-reactivity or interference between Human ST2 and analogues was observed.
