The Human sSele (Soluble E-selectin) ELISA Kit is specifically designed for the precise measurement of soluble E-selectin levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.E-selectin is a key adhesion molecule involved in inflammation and immune response, playing a critical role in the recruitment of leukocytes to sites of infection or injury.
Elevated levels of soluble E-selectin have been linked to various inflammatory conditions, including cardiovascular diseases, autoimmune disorders, and diabetes, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human sSele (Soluble E-selectin) ELISA Kit offers reliable and reproducible results, making it an essential tool for researchers investigating the role of E-selectin in health and disease.
Product Name:
Human sSELE (Soluble E-Selectin) ELISA Kit
SKU:
HUES02004
Target:
Human sSELE (Soluble E-Selectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Selectin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human E-Selectin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human E-Selectin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human E-Selectin. You can calculate the concentration of Human E-Selectin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
91-106
85-99
Average (%)
96
97
91
1:4
Range (%)
95-110
85-98
95-107
Average (%)
101
89
101
1:8
Range (%)
94-111
86-100
99-114
Average (%)
101
92
105
1:16
Range (%)
92-106
81-93
91-108
Average (%)
100
88
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
274.4
734.9
2238.8
279.9
759.1
2430.2
Standard deviation
16.2
41.2
109.7
17.9
42.5
116.6
C V (%)
5.9
5.61
4.9
6.4
5.6
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human E-Selectin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human E-Selectin in samples. No significant cross-reactivity or interference between Human E-Selectin and analogues was observed.
