Human SRD5a1 (Steroid 5 Alpha Reductase 1) ELISA Kit
The Human SRD5A1 (Steroid 5 Alpha-Reductase 1) ELISA Kit is specifically designed for the accurate detection of SRD5A1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results that are essential for various research applications.SRD5A1 is an important enzyme involved in the conversion of testosterone to dihydrotestosterone (DHT), a potent androgen hormone. Dysregulation of SRD5A1 has been linked to conditions such as prostate cancer, male-pattern baldness, and hirsutism.
Therefore, accurate measurement of SRD5A1 levels is crucial for understanding these conditions and developing targeted therapies.By using the Human SRD5A1 ELISA Kit, researchers can effectively study the role of SRD5A1 in various physiological and pathological processes, leading to advancements in the fields of endocrinology, oncology, and dermatology. Trust in the reliability and accuracy of this kit to support your research needs and drive innovative discoveries.
Product Name:
Human SRD5a1 (Steroid 5 Alpha Reductase 1) ELISA Kit
SKU:
HUES03368
Target:
Human SRD5a1 (Steroid 5 Alpha Reductase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SRD5a1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SRD5a1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SRD5a1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SRD5a1. You can calculate the concentration of Human SRD5a1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
90-103
90-101
Average (%)
90
96
96
1:4
Range (%)
91-103
81-96
83-95
Average (%)
96
88
90
1:8
Range (%)
92-106
84-93
84-97
Average (%)
99
88
89
1:16
Range (%)
92-106
80-90
85-100
Average (%)
99
85
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.03
189.06
923.43
103.68
202.45
859.14
Standard deviation
5.66
7.64
38.78
5.22
9.31
36.26
C V (%)
5.29
4.04
4.2
5.03
4.6
4.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SRD5a1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SRD5a1 in samples. No significant cross-reactivity or interference between Human SRD5a1 and analogues was observed.
