Human SPINK1 Recombinant Protein (RPPB2280)

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SKU:
RPPB2280
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
SPINK1
Synonyms:
Serine Peptidase Inhibitor Kazal Type 1
Synonyms:
Pancreatic Secretory Trypsin Inhibitor
Synonyms:
Tumor-Associated Trypsin Inhibitor
Synonyms:
PCTT
Source:
Escherichia Coli
Uniprot:
P00995
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Description

Product Name:Human SPINK1 Recombinant Protein
Product Code:RPPB2280
Size:20µg
Species:Human
Target:SPINK1
Synonyms:Serine Peptidase Inhibitor Kazal Type 1, Pancreatic Secretory Trypsin Inhibitor, Tumor-Associated Trypsin Inhibitor, PCTT, TATI, Spink3, Serine Protease Inhibitor Kazal Type 1, TCP, PSTI.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The SPINK1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSDSLGREA KCYNELNGCT KIYDPVCGTD GNTYPNECVL CFENRKRQTS ILIQKSGPC

Trypsin inhibitor SPINK1 is secreted from pancreatic acinar cells into pancreatic fluid. SPINK1 has a role in inhibition of trypsin-catalyzed premature initiation of zymogens in the pancreas and the pancreatic duct. Alterations in SPINK1 gene are the cause for tropical calcific and pancreatitis hereditary pancreatitis.

SPINK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 79 amino acids (24-79) and having a molecular mass of 8.6kDa.SPINK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SPINK1: This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: extracellular space

Molecular Function:serine-type endopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction

Disease: Tropical Calcific Pancreatitis; Pancreatitis, Hereditary

NCBI Summary:The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]
UniProt Code:P00995
NCBI GenInfo Identifier:124856
NCBI Gene ID:6690
NCBI Accession:P00995.2
UniProt Related Accession:P00995
Molecular Weight:79
NCBI Full Name:Pancreatic secretory trypsin inhibitor
NCBI Synonym Full Names:serine peptidase inhibitor, Kazal type 1
NCBI Official Symbol:SPINK1  
NCBI Official Synonym Symbols:TCP; PCTT; PSTI; TATI; Spink3  
NCBI Protein Information:pancreatic secretory trypsin inhibitor; tumor-associated trypsin inhibitor; serine protease inhibitor, Kazal type 1
UniProt Protein Name:Pancreatic secretory trypsin inhibitor
UniProt Synonym Protein Names:Serine protease inhibitor Kazal-type 1; Tumor-associated trypsin inhibitor; TATI
Protein Family:Serine protease inhibitor
UniProt Gene Name:SPINK1  
UniProt Entry Name:ISK1_HUMAN
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