Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
The Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit is specifically designed for the precise quantification of SPD levels in human samples, including serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and consistent results, making it well-suited for various research applications.SPD is a key protein involved in pulmonary surfactant function, playing a crucial role in maintaining lung integrity and function.
Changes in SPD levels have been associated with various respiratory disorders, making it a valuable biomarker for studying lung diseases and developing potential therapeutic interventions.Overall, the Human SPD ELISA Kit provides researchers with a reliable tool for investigating the role of SPD in pulmonary health and disease, offering valuable insights for advancing our understanding and treatment of respiratory conditions.
Product Name:
Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
SKU:
HUES02327
Target:
Human SPD (Pulmonary Surfactant Associated Protein D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SP-D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SP-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SP-D. You can calculate the concentration of Human SP-D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
92-107
98-111
Average (%)
95
97
105
1:4
Range (%)
90-107
86-100
84-96
Average (%)
98
92
90
1:8
Range (%)
87-102
83-96
86-100
Average (%)
93
89
94
1:16
Range (%)
87-98
80-92
86-102
Average (%)
93
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
83-96
90
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.09
10.52
47.37
4.93
11.41
50.39
Standard deviation
0.29
0.44
2.27
0.25
0.5
2.46
C V (%)
5.7
4.18
4.79
5.07
4.38
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SP-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SP-D in samples. No significant cross-reactivity or interference between Human SP-D and analogues was observed.
