Human SPC (Pulmonary Surfactant Associated Protein C) CLIA Kit
The Human SPC (Pulmonary Surfactant-Associated Protein C) CLIA Kit is specially designed for the precise measurement of SPC levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an indispensable tool for various research applications.SPC is a vital protein that plays a crucial role in the function of pulmonary surfactant, which is essential for maintaining the proper function of the lungs and preventing respiratory distress.
Abnormalities in SPC levels have been linked to respiratory diseases such as acute respiratory distress syndrome (ARDS) and other lung disorders.By accurately measuring SPC levels, researchers can gain valuable insights into the role of this protein in lung function and disease pathology. This kit provides a reliable and efficient way to study pulmonary diseases and develop potential therapeutic interventions for improving lung health.
Product Name:
Human SPC (Pulmonary Surfactant Associated Protein C) CLIA Kit
SKU:
HUES00725
Target:
Human SPC (Pulmonary Surfactant Associated Protein C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SPC. You can calculate the concentration of Human SPC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
89-101
97-112
Average (%)
98
96
103
1:4
Range (%)
98-111
96-111
86-99
Average (%)
105
104
91
1:8
Range (%)
96-111
90-103
86-99
Average (%)
103
97
92
1:16
Range (%)
95-108
97-113
98-110
Average (%)
103
105
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.21
7.45
20.26
3.05
7.39
18.94
Standard deviation
0.38
0.82
1.89
0.36
0.59
1.31
C V (%)
11.84
11.01
9.33
11.8
7.98
6.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SPC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPC in samples. No significant cross-reactivity or interference between Human SPC and analogues was observed.
