Human SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
The Human SP-B (Pulmonary Surfactant Associated Protein B) ELISA Kit is specifically designed for the accurate measurement of SP-B levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.SP-B is a key protein found in pulmonary surfactant, essential for maintaining proper lung function and preventing alveolar collapse.
Dysregulation of SP-B has been linked to various respiratory conditions such as acute respiratory distress syndrome (ARDS) and respiratory distress syndrome (RDS) in newborns. Therefore, this ELISA kit serves as a valuable tool for studying these conditions and developing potential therapeutic interventions.
Product Name:
Human SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
SKU:
HUES02325
Target:
Human SPB (Pulmonary Surfactant Associated Protein B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SP-B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SP-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SP-B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SP-B. You can calculate the concentration of Human SP-B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
96-107
87-100
Average (%)
93
102
93
1:4
Range (%)
91-107
81-93
89-102
Average (%)
98
88
94
1:8
Range (%)
90-101
83-93
84-98
Average (%)
95
88
89
1:16
Range (%)
86-98
82-95
84-95
Average (%)
92
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.42
7.23
22.89
2.26
7.33
20.64
Standard deviation
0.16
0.35
0.71
0.12
0.41
1.05
C V (%)
6.61
4.84
3.1
5.31
5.59
5.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SP-B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SP-B in samples. No significant cross-reactivity or interference between Human SP-B and analogues was observed.
