Human SPB (Pulmonary Surfactant Associated Protein B) CLIA Kit
The Human SPB (Pulmonary Surfactant Associated Protein B) CLIA Kit is a specialized assay designed for the precise and accurate measurement of SPB levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it a valuable tool for various research applications.SPB is a vital protein involved in the function of pulmonary surfactant, which is essential for maintaining the structural integrity of the lungs and facilitating proper gas exchange.
Abnormalities in SPB levels have been linked to respiratory diseases such as acute respiratory distress syndrome (ARDS) and infant respiratory distress syndrome (IRDS), highlighting the importance of monitoring SPB levels for early detection and management of these conditions.Whether studying lung development, respiratory diseases, or exploring potential therapeutic targets, the Human SPB CLIA Kit provides researchers with a reliable and accurate method for quantifying SPB levels, advancing our understanding of pulmonary health and disease.
Product Name:
Human SPB (Pulmonary Surfactant Associated Protein B) CLIA Kit
SKU:
HUES00724
Target:
Human SPB (Pulmonary Surfactant Associated Protein B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SPB. You can calculate the concentration of Human SPB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
86-97
92-102
Average (%)
102
91
97
1:4
Range (%)
87-100
102-120
97-107
Average (%)
94
109
102
1:8
Range (%)
87-99
98-113
85-96
Average (%)
92
106
91
1:16
Range (%)
87-98
102-114
91-108
Average (%)
93
108
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
90
EDTA plasma (n=5)
98-113
104
Cell culture media (n=5)
95-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.13
9.37
43.13
4.93
9.37
43.98
Standard deviation
0.62
0.67
3.06
0.41
1.12
3.47
C V (%)
12.09
7.15
7.09
8.32
11.95
7.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SPB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPB in samples. No significant cross-reactivity or interference between Human SPB and analogues was observed.
