Human SOX18 (Sex Determining Region Y Box Protein 18) CLIA Kit
The Human SOX18 (Sex-Determining Region Y-Box Protein 18) CLIA Kit is a specialized assay designed for the quantitative detection of SOX18 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SOX18 is a transcription factor that plays a crucial role in embryonic development and is involved in regulating the formation of blood vessels and lymphatic vessels.
Dysregulation of SOX18 has been linked to various diseases, including cardiovascular disorders, lymphedema, and certain cancers, making it a valuable biomarker for studying these conditions and potential therapeutic targets.Overall, the Human SOX18 CLIA Kit from AssayGenie provides researchers with a reliable tool for the quantitative analysis of SOX18 levels, allowing for in-depth exploration of its role in health and disease.
Product Name:
Human SOX18 (Sex Determining Region Y Box Protein 18) CLIA Kit
SKU:
HUES01133
Target:
Human SOX18 (Sex Determining Region Y Box Protein 18)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SOX18. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOX18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOX18, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SOX18. You can calculate the concentration of Human SOX18 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
99-114
98-111
Average (%)
104
105
105
1:4
Range (%)
100-114
100-119
89-104
Average (%)
107
108
96
1:8
Range (%)
89-100
85-100
98-112
Average (%)
95
91
106
1:16
Range (%)
103-119
97-110
86-98
Average (%)
109
103
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
94
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.41
174.44
900.43
98.92
160.5
959.78
Standard deviation
9.43
13.01
88.96
9.08
13.53
108.94
C V (%)
9.21
7.46
9.88
9.18
8.43
11.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SOX18 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SOX18 in samples. No significant cross-reactivity or interference between Human SOX18 and analogues was observed.
