The Human SOST (Sclerostin) ELISA Kit is specifically designed to measure Sclerostin levels in human samples including serum, plasma, and cell culture supernatants. This kit provides highly accurate and precise results, ensuring reliable data for various research applications.Sclerostin is a key regulator of bone metabolism, inhibiting bone formation by blocking the Wnt signaling pathway. Elevated levels of Sclerostin have been associated with conditions such as osteoporosis, making it a vital biomarker for studying bone health and developing new treatments.
With its high sensitivity and specificity, the Human SOST (Sclerostin) ELISA Kit is an indispensable tool for researchers investigating the role of Sclerostin in bone biology and exploring potential therapeutic interventions for bone-related disorders.
Product Name:
Human SOST (Sclerostin) ELISA Kit
SKU:
HUES02558
Target:
Human SOST (Sclerostin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SOST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SOST. You can calculate the concentration of Human SOST in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
94-108
89-102
Average (%)
92
100
96
1:4
Range (%)
92-104
82-94
86-97
Average (%)
98
87
91
1:8
Range (%)
88-102
82-93
84-94
Average (%)
94
88
89
1:16
Range (%)
87-101
85-100
86-99
Average (%)
94
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
222.11
370.69
1524.64
203.0
389.31
1557.6
Standard deviation
11.82
15.05
67.24
11.27
21.3
67.6
C V (%)
5.32
4.06
4.41
5.55
5.47
4.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SOST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed.
