The Human SORT1 (Sortilin-1) ELISA Kit is specifically designed for the precise measurement of sortilin-1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research experiments.Sortilin-1 is a key protein involved in various cellular processes, including protein trafficking and cell signaling. Dysregulation of sortilin-1 has been associated with several diseases, including Alzheimer's disease, atherosclerosis, and metabolic disorders, making it a valuable biomarker for understanding these conditions and exploring potential treatment options.
For researchers looking to investigate the role of sortilin-1 in disease pathogenesis or explore its therapeutic potential, the Human SORT1 ELISA Kit provides a powerful tool for precise and reliable protein quantification.
Product Name:
Human SORT1 (Sortilin 1) ELISA Kit
SKU:
HUES03417
Target:
Human SORT1 (Sortilin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SORT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SORT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SORT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SORT1. You can calculate the concentration of Human SORT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
91-108
95-109
Average (%)
96
98
100
1:4
Range (%)
90-102
81-92
85-97
Average (%)
95
87
91
1:8
Range (%)
92-106
83-93
87-100
Average (%)
99
88
93
1:16
Range (%)
92-104
85-97
86-100
Average (%)
99
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
94-106
101
Cell culture media (n=5)
88-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.2
3.82
0.5
1.24
3.84
Standard deviation
0.04
0.06
0.15
0.03
0.05
0.21
C V (%)
7.41
5.0
3.93
6.0
4.03
5.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SORT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SORT1 in samples. No significant cross-reactivity or interference between Human SORT1 and analogues was observed.
