The Human SNC (Alpha-Synuclein) Alpha ELISA Kit is specifically designed for the accurate and sensitive detection of alpha-synuclein levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Alpha-synuclein is a key protein involved in neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease, and dementia with Lewy bodies. Abnormal accumulation of alpha-synuclein in the brain is associated with the development and progression of these conditions, making it a valuable biomarker for studying disease pathology and potential therapeutic interventions.
With its high performance and ease of use, the Human SNC (Alpha-Synuclein) Alpha ELISA Kit is an essential tool for researchers and clinicians looking to further understand the role of alpha-synuclein in neurodegenerative diseases, paving the way for new diagnostic and treatment strategies.
Product Name:
Human SNC alpha (Synuclein Alpha) ELISA Kit
SKU:
HUES02100
Target:
Human SNC alpha (Synuclein Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SNCα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SNCα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SNCα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SNCα. You can calculate the concentration of Human SNCα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
85-97
90-104
Average (%)
105
90
96
1:4
Range (%)
90-102
84-99
87-98
Average (%)
97
91
93
1:8
Range (%)
89-100
81-96
83-95
Average (%)
94
87
88
1:16
Range (%)
94-105
86-99
83-98
Average (%)
100
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.06
88.69
424.69
52.94
92.49
393.01
Standard deviation
3.33
5.15
13.17
3.48
4.8
19.02
C V (%)
6.79
5.81
3.1
6.57
5.19
4.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SNCα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SNCα in samples. No significant cross-reactivity or interference between Human SNCα and analogues was observed.
