Human Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7) ELISA Kit
The Human SMAD7 (MADH7, Mothers Against Decapentaplegic Homolog 7) ELISA Kit is a reliable tool for the precise measurement of SMAD7 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for various research applications.SMAD7 is a key protein in the TGF-beta signaling pathway, where it acts as a negative regulator, inhibiting the effects of TGF-beta on cell growth and differentiation.
Dysregulation of SMAD7 has been implicated in numerous diseases such as cancer, fibrosis, and inflammatory disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human SMAD7 ELISA Kit offers researchers a reliable tool for studying the role of SMAD7 in various diseases and advancing our understanding of TGF-beta signaling pathways.
Product Name:
Human Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7) ELISA Kit
SKU:
HUES02058
Target:
Human Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Smad7/MADH7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad7/MADH7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad7/MADH7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Smad7/MADH7. You can calculate the concentration of Human Smad7/MADH7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
90-103
96-112
Average (%)
99
95
102
1:4
Range (%)
93-106
86-99
82-94
Average (%)
99
91
87
1:8
Range (%)
92-105
79-92
87-99
Average (%)
99
85
92
1:16
Range (%)
93-107
84-100
82-92
Average (%)
98
91
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.04
3.81
0.49
1.02
3.95
Standard deviation
0.03
0.06
0.16
0.03
0.05
0.16
C V (%)
5.77
5.77
4.2
6.12
4.9
4.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Smad7/MADH7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Smad7/MADH7 in samples. No significant cross-reactivity or interference between Human Smad7/MADH7 and analogues was observed.
