The Human SLIT2 (Slit Homolog 2) ELISA Kit is specifically designed for the precise measurement of SLIT2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research purposes.SLIT2 is a key protein involved in axon guidance and cell migration, playing a crucial role in neurological development and other physiological processes. Dysregulation of SLIT2 expression has been implicated in conditions such as cancer, developmental disorders, and autoimmune diseases, highlighting its importance as a biomarker for further research and therapeutic development.
Overall, the Human SLIT2 ELISA Kit from Assay Genie provides researchers with a powerful tool to investigate the role of SLIT2 in various biological processes and diseases, ultimately contributing to advancements in understanding and treating related conditions.
Product Name:
Human Slit2 (Slit Homolog 2) ELISA Kit
SKU:
HUES02055
Target:
Human Slit2 (Slit Homolog 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Slit2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Slit2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Slit2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Slit2. You can calculate the concentration of Human Slit2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
91-105
93-106
Average (%)
100
96
101
1:4
Range (%)
88-103
87-100
82-95
Average (%)
96
92
88
1:8
Range (%)
91-103
82-94
88-101
Average (%)
97
87
93
1:16
Range (%)
93-109
83-95
87-101
Average (%)
99
88
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.22
4.57
0.5
1.33
4.91
Standard deviation
0.03
0.07
0.24
0.03
0.06
0.26
C V (%)
6.25
5.74
5.25
6.0
4.51
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Slit2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Slit2 in samples. No significant cross-reactivity or interference between Human Slit2 and analogues was observed.
