Human SLC (Secondary Lymphoid Tissue Chemokine) CLIA Kit
The Human SLC (Secondary Lymphoid Tissue Chemokine) CLIA Kit is specifically designed for the precise quantification of SLC levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for researchers in various fields.SLC, also known as CCL21, plays a critical role in immune cell trafficking and the regulation of immune responses. Dysregulation of SLC levels has been linked to various inflammatory and autoimmune diseases, highlighting its importance as a potential biomarker for diagnostic and therapeutic purposes.
By utilizing the Human SLC CLIA Kit, researchers can analyze SLC levels in a quick and efficient manner, allowing for a deeper understanding of its role in disease pathogenesis and potentially identifying new therapeutic targets. Stay ahead in your research with this advanced kit from AssayGenie.
Product Name:
Human SLC (Secondary Lymphoid Tissue Chemokine) CLIA Kit
SKU:
HUES00028
Target:
Human SLC (Secondary Lymphoid Tissue Chemokine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SLC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SLC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SLC. You can calculate the concentration of Human SLC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
88-103
98-113
Average (%)
108
96
103
1:4
Range (%)
86-99
87-98
98-114
Average (%)
93
92
104
1:8
Range (%)
102-118
84-97
86-101
Average (%)
110
91
93
1:16
Range (%)
99-115
88-102
100-111
Average (%)
107
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
105
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.97
200.41
874.2
99.02
195.18
955.07
Standard deviation
10.41
20.58
95.38
8.17
14.97
60.65
C V (%)
10.85
10.27
10.91
8.25
7.67
6.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SLC in samples. No significant cross-reactivity or interference between Human SLC and analogues was observed.
