Human SIGLEC2 (Sialic Acid Binding Ig Like Lectin 2) CLIA Kit
The Human Siglec2 (Sialic Acid Binding Ig-like Lectin 2) CLIA Kit is a reliable and sensitive assay designed for the accurate detection of Siglec2 levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, providing researchers with precise and consistent results for a variety of research applications.Siglec2 is a cell surface protein known for its role in regulating immune responses and inflammation. It binds to sialic acid molecules on cell surfaces, modulating immune cell activation and function.
Dysregulation of Siglec2 has been implicated in autoimmune diseases and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic targets.The Human Siglec2 CLIA Kit from Assay Genie offers researchers a robust tool for investigating the role of Siglec2 in health and disease, providing valuable insights into immune regulation and potential therapeutic interventions.
Product Name:
Human SIGLEC2 (Sialic Acid Binding Ig Like Lectin 2) CLIA Kit
SKU:
HUES00764
Target:
Human SIGLEC2 (Sialic Acid Binding Ig Like Lectin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SIGLEC2. You can calculate the concentration of Human SIGLEC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
90-102
89-102
Average (%)
105
97
95
1:4
Range (%)
99-113
94-106
86-97
Average (%)
106
100
92
1:8
Range (%)
87-100
99-113
92-103
Average (%)
93
104
98
1:16
Range (%)
100-116
87-100
91-104
Average (%)
107
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
97-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.67
126.43
278.01
39.49
136.68
263.32
Standard deviation
3.47
14.84
22.27
4.77
10.03
27.99
C V (%)
8.33
11.74
8.01
12.08
7.34
10.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SIGLEC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SIGLEC2 in samples. No significant cross-reactivity or interference between Human SIGLEC2 and analogues was observed.
