Single Ig IL-1-related receptor (SIGIRR) or TIR8 is a member of Toll-like receptor-interleukin 1 receptor signaling (TLR-IL-1R) receptor superfamily. Although SIGIRR/TIR8 shows the typical conserved motifs that characterize the IL-1R and Toll superfamily; it is structurally and functionally distinct from both. SIGIRR/TIR8 has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll; yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR/TIR8 was mainly expressed in mouse and human epithelial tissues such as kidney; lung and gut. Resting and activated T and B lymphocytes and monocytes-macrophages expressed little or no SIGIRR/TIR8; with the exception of the mouse GG2EE macrophage line. Inflammation is enhanced in SIGIRR-deficient mice. SIGIRR negatively modulates immune responses. Inflammation is enhanced in SIGIRR-deficient mice; as shown by their enhanced chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge.
Product Name:
Human SIGIRR/TIR8 Recombinant Protein (RPES3314)
Product Code:
RPES3314
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
TIR8
Accession:
Q6IA17-1
Sequence:
Met 1-His 118
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human SIGIRR (Q6IA17-1) extracellular domain (Met 1-His 118) was expressed; fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
14 kDa
AP Mol Mass:
25-35 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
