Surfactant Pulmonary-Associated Protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that places it in a subset of pattern recognition proteins termed defense collagens. SP-D is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures and induced in cardiac smooth muscle and endothelial cells. It binds both secreted and transmembrane proteins that transduce its function. It binds human neutrophil defensins, modulating influenza anti-viral defense. It binds MD-2/LY96, a secreted protein that cooperates with Toll-like receptors (TLRs) in the response of macrophages to bacterial lipopolysaccharides (LPS) or cell wall components. It also binds macrophage CD14 and TLRs directly, blocking binding of LPS and down-regulating TNF-? secretion. SP-D binding of both SIRP? and the calreticulin/CD91 complex on macrophages allows for a graded response to environmental challenge.
Product Name:
Human SFTPD/SP-D Recombinant Protein (RPES3547)
Product Code:
RPES3547
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Pulmonary Surfactant-Associated Protein D, PSP-D, SP-D, Collectin-7, Lung Surfactant Protein D, SFTPD, COLEC7, PSPD, SFTP4,COLEC7,SFTP4
Accession:
P35247
Sequence:
Ala21-Phe375(Glu22Gly)
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Pulmonary Surfactant-Associated Protein D is produced by our Mammalian expression system and the target gene encoding Ala21-Phe375(Glu22Gly)? is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
36.5 kDa
AP Mol Mass:
45 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
