SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Product Name:
Human SerpinD1 Recombinant Protein (RPES4668)
Product Code:
RPES4668
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
D22S673,HC2,HCF2,HCII,HLS2,LS2,SerpinD1,THPH10
Accession:
NP_000176.2
Sequence:
Met 1-Ser 499
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.Immobilized recombinant human SerpinD1-His at 10 µg/ml (100 ?l/well) can bind biotinylated recombinant mouse ELANE-His with a linear range of 0.15-10.0 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human SerpinD1 precursor (NP_000176.2) (Met 1-Ser 499) was expressed with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
56.4 kDa
AP Mol Mass:
65-70 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
