The Human SEPP1 (Selenoprotein P) Plasma ELISA Kit is specifically designed for the quantitative detection of SEPP1 levels in human plasma samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.SEPP1 is a key selenoprotein involved in the transport and regulation of selenium in the body, playing a critical role in antioxidant defense and immune function. Abnormal SEPP1 levels have been associated with various health conditions, including thyroid disease, cardiovascular diseases, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With easy-to-follow protocols and efficient performance, the Human SEPP1 Plasma ELISA Kit from Assay Genie is an essential tool for researchers looking to explore the role of SEPP1 in health and disease.
Product Name:
Human SEPP1 (Selenoprotein P, Plasma) ELISA Kit
SKU:
HUES03076
Target:
Human SEPP1 (Selenoprotein P, Plasma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEPP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEPP1. You can calculate the concentration of Human SEPP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
89-101
96-112
Average (%)
92
94
102
1:4
Range (%)
88-104
82-96
86-98
Average (%)
95
88
91
1:8
Range (%)
86-98
83-96
82-96
Average (%)
93
88
89
1:16
Range (%)
91-102
83-94
81-95
Average (%)
96
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
95-109
100
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.35
3.52
0.51
1.38
3.78
Standard deviation
0.03
0.06
0.12
0.03
0.07
0.2
C V (%)
6.25
4.44
3.41
5.88
5.07
5.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SEPP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SEPP1 in samples. No significant cross-reactivity or interference between Human SEPP1 and analogues was observed.
