Human SEPCR/PROCR (Soluble Endothelial Protein C Receptor) CLIA Kit
The Human sEPCR (Soluble Endothelial Protein C Receptor) CLIA Kit is a highly sensitive and specific assay designed to accurately detect levels of soluble endothelial protein C receptor in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it a valuable tool for research in various fields.sEPCR is a key protein involved in regulating blood coagulation and inflammation, playing a crucial role in endothelial function and vascular health.
Dysregulation of sEPCR has been linked to various diseases such as sepsis, thrombosis, and cardiovascular disorders, making it a promising biomarker for understanding these conditions and developing targeted therapies.With its high performance and versatility, the Human sEPCR CLIA Kit from Assay Genie is an essential tool for researchers looking to study the role of soluble endothelial protein C receptor in health and disease.
Product Name:
Human SEPCR/PROCR (Soluble Endothelial Protein C Receptor) CLIA Kit
SKU:
HUES01221
Target:
Human SEPCR/PROCR (Soluble Endothelial Protein C Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human sEPCR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sEPCR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sEPCR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human sEPCR. You can calculate the concentration of Human sEPCR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
93-105
100-116
Average (%)
102
99
108
1:4
Range (%)
97-111
85-96
98-112
Average (%)
104
91
104
1:8
Range (%)
86-99
100-119
99-115
Average (%)
92
108
108
1:16
Range (%)
97-112
85-98
94-107
Average (%)
102
91
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
107
EDTA plasma (n=5)
100-114
107
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.52
3.49
0.52
1.5
3.52
Standard deviation
0.05
0.17
0.24
0.06
0.12
0.22
C V (%)
10.0
11.18
6.88
11.54
8.0
6.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human sEPCR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sEPCR in samples. No significant cross-reactivity or interference between Human sEPCR and analogues was observed.
