Human SEMG1 Recombinant Protein (RPPB4616)
- SKU:
- RPPB4616
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- SEMG1
- Synonyms:
- CT103
- dJ172H202
- SEMG
- SGI
- Source:
- Escherichia Coli
- Uniprot:
- P04279
Description
| Product Name: | Human SEMG1 Recombinant Protein |
| Product Code: | RPPB4616 |
| Size: | 20µg |
| Species: | Human |
| Target: | SEMG1 |
| Synonyms: | CT103, dJ172H20.2, SEMG, SGI, Semenogelin-1, Semenogelin I, SEMG1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The SEMG1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.5M NaCl, 10% glycerol, 250mM Imidazole, 0.1mM PMSF and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT |
SEMG1 is the predominant protein in semen. The secreted protein takes part in the formation of a gel matrix which encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes SEMG1 into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to shift more freely. 2 transcript variants encoding different isoforms have been found for SEMG1.
SEMG1 Human Recombinant produced in E. coli is a single polypeptide chain containing 462 amino acids (24-462) and having a molecular mass of 52kDa. SEMG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SEMG1: Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Belongs to the semenogelin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cancer Testis Antigen (CTA); Secreted Chromosomal Location of Human Ortholog: 20q12-q13.2 Cellular Component: extracellular region; extracellular space; nucleus; protein complex Molecular Function:metal ion binding; protein binding Biological Process: antibacterial humoral response; cellular protein metabolic process; coagulation; insemination; protein heterooligomerization |
| NCBI Summary: | The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P04279 |
| NCBI GenInfo Identifier: | 134426 |
| NCBI Gene ID: | 6406 |
| NCBI Accession: | P04279.2 |
| UniProt Secondary Accession: | P04279,Q53ZV0, Q53ZV1, Q53ZV2, Q6X4I9, Q6Y809, Q6Y822 Q6Y823, Q86U64, Q96QM3, |
| UniProt Related Accession: | P04279 |
| Molecular Weight: | 45,322 Da |
| NCBI Full Name: | Semenogelin-1 |
| NCBI Synonym Full Names: | semenogelin I |
| NCBI Official Symbol: | SEMG1 |
| NCBI Official Synonym Symbols: | SGI; SEMG; CT103; dJ172H20.2 |
| NCBI Protein Information: | semenogelin-1 |
| UniProt Protein Name: | Semenogelin-1 |
| UniProt Synonym Protein Names: | Cancer/testis antigen 103; Semenogelin I; SGI |
| Protein Family: | Semenogelin |
| UniProt Gene Name: | SEMG1 |
| UniProt Entry Name: | SEMG1_HUMAN |
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