Human SEMA7A Recombinant Protein (RPPB4615)
- SKU:
- RPPB4615
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Human
- Target:
- SEMA7A
- Synonyms:
- Semaphorin-7A
- CDw108
- JMH blood group antigen
- John-Milton-Hargen human blood group Ag
- Source:
- Sf9 Insect cells
- Uniprot:
- O75326
Description
| Product Name: | Human SEMA7A Recombinant Protein |
| Product Code: | RPPB4615 |
| Size: | 5µg |
| Species: | Human |
| Target: | SEMA7A |
| Synonyms: | Semaphorin-7A, CDw108, JMH blood group antigen, John-Milton-Hargen human blood group Ag, Semaphorin-K1, Sema K1, Semaphorin-L, Sema L, CD108. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | SEMA7A protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPQGHLRSG PRIFAVWKGH VGQDRVDFGQ TEPHTVLFHE PGSSSVWVGG RGKVYLFDFP EGKNASVRTV NIGSTKGSCL DKRDCENYIT LLERRSEGLL ACGTNARHPS CWNLVNGTVV PLGEMRGYAP FSPDENSLVL FEGDEVYSTI RKQEYNGKIP RFRRIRGESE LYTSDTVMQN PQFIKATIVH QDQAYDDKIY YFFREDNPDK NPEAPLNVSR VAQLCRGDQG GESSLSVSKW NTFLKAMLVC SDAATNKNFN RLQDVFLLPD PSGQWRDTRV YGVFSNPWNY SAVCVYSLGD IDKVFRTSSL KGYHSSLPNP RPGKCLPDQQ PIPTETFQVA DRHPEVAQRV EPMGPLKTPL FHSKYHYQKV AVHRMQASHG ETFHVLYLTT DRGTIHKVVE PGEQEHSFAF NIMEIQPFRR AAAIQTMSLD AERRKLYVSS QWEVSQVPLD LCEVYGGGCH GCLMSRDPYC GWDQGRCISI YSSERSVLQS INPAEPHKEC PNPKPDKAPL QKVSLAPNSR YYLSCPMESR HATYSWRHKE NVEQSCEPGH QSPNCILFIE NLTAQQYGHY FCEAQEGSYF REAQHWQLLP EDGIMAEHLL GHACALALEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH |
SEMA7A (semaphorin-7A isoform 1), is a membrane-bound semaphorin which is linked with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. SEMA7A encourages formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK3 & MAPK1. SEMA7A takes a vital part in integrin-mediated signaling and functions in regulating cell migration as well as immune responses. In addition, SEMA7A promotes the production of proinflammatory cytokines by macrophages & monocytes.
SEMA7A produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 846 amino acids (45-648 a.a.) and having a molecular mass of 95.7kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).SEMA7A is expressed with an 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SEMA7A: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Belongs to the semaphorin family. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 15q22.3-q23 Cellular Component: external side of plasma membrane; extracellular space; membrane; plasma membrane Molecular Function:chemorepellent activity; integrin binding; protein binding; semaphorin receptor binding Biological Process: immune response; integrin-mediated signaling pathway; negative chemotaxis; negative regulation of axon extension involved in axon guidance; neural crest cell migration; osteoblast differentiation; positive regulation of axon extension; positive regulation of cell migration; regulation of inflammatory response Disease: Blood Group, John Milton Hagen System |
| NCBI Summary: | This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
| UniProt Code: | O75326 |
| NCBI GenInfo Identifier: | 14548264 |
| NCBI Gene ID: | 8482 |
| NCBI Accession: | O75326.1 |
| UniProt Secondary Accession: | O75326,Q1XE81, Q1XE82, Q1XE83, Q1XE84, Q3MIY5, B4DDP7 F5H1S0, |
| UniProt Related Accession: | O75326 |
| Molecular Weight: | 73,364 Da |
| NCBI Full Name: | Semaphorin-7A |
| NCBI Synonym Full Names: | semaphorin 7A (John Milton Hagen blood group) |
| NCBI Official Symbol: | SEMA7A |
| NCBI Official Synonym Symbols: | JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1 |
| NCBI Protein Information: | semaphorin-7A |
| UniProt Protein Name: | Semaphorin-7A |
| UniProt Synonym Protein Names: | CDw108; JMH blood group antigen; John-Milton-Hargen human blood group Ag; Semaphorin-K1; Sema K1; Semaphorin-L; Sema L; CD_antigen: CD108 |
| Protein Family: | Semaphorin |
| UniProt Gene Name: | SEMA7A |
| UniProt Entry Name: | SEM7A_HUMAN |
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