The Human SEMA4D (Semaphorin 4D) ELISA Kit is specifically designed for the accurate detection of SEMA4D levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it ideal for a variety of research applications.SEMA4D is a critical protein that plays a key role in immune response modulation, angiogenesis regulation, and tumor growth. It is implicated in various diseases such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.
With its reliable performance and versatility, the Human SEMA4D (Semaphorin 4D) ELISA Kit is an essential tool for researchers seeking to investigate the role of SEMA4D in disease pathology and explore its potential as a therapeutic target.
Product Name:
Human SEMA4D (Semaphorin 4D) ELISA Kit
SKU:
HUES03137
Target:
Human SEMA4D (Semaphorin 4D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA4D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA4D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEMA4D. You can calculate the concentration of Human SEMA4D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
93-108
101-115
Average (%)
100
99
106
1:4
Range (%)
91-104
83-95
88-100
Average (%)
96
90
94
1:8
Range (%)
87-102
81-92
86-97
Average (%)
93
86
92
1:16
Range (%)
88-100
80-92
83-98
Average (%)
95
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
2.48
9.92
0.96
2.31
10.06
Standard deviation
0.07
0.14
0.46
0.06
0.1
0.45
C V (%)
6.67
5.65
4.64
6.25
4.33
4.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SEMA4D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SEMA4D in samples. No significant cross-reactivity or interference between Human SEMA4D and analogues was observed.
