Human sdLDL (Small Dense Low Density Lipoprotein) ELISA Kit
The Human sdLDL (small dense low-density lipoprotein) ELISA Kit is specifically designed for the precise quantification of sdLDL levels in human serum samples. This kit offers high sensitivity and accuracy, allowing for reliable and consistent results in various research settings.sdLDL is a subtype of LDL cholesterol characterized by smaller particle size and higher density, making it more atherogenic and potentially increasing the risk of cardiovascular diseases. Monitoring sdLDL levels can provide valuable insights into cardiovascular health and help in the assessment of cardiovascular disease risk.
With its easy-to-use format and comprehensive instructions, the Human sdLDL ELISA Kit is an essential tool for researchers studying lipid metabolism, atherosclerosis, and cardiovascular diseases. Its reliable performance and precise measurements make it an indispensable asset for advancing research in the field of cardiovascular health.
Product Name:
Human sdLDL (Small Dense Low Density Lipoprotein) ELISA Kit
SKU:
HUES03392
Target:
Human sdLDL (Small Dense Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 nmol/mL
Detection range:
0.31-20 nmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sdLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sdLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sdLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sdLDL. You can calculate the concentration of Human sdLDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
94-105
98-113
Average (%)
97
100
105
1:4
Range (%)
90-104
80-93
86-99
Average (%)
95
86
91
1:8
Range (%)
93-110
80-93
88-100
Average (%)
100
85
93
1:16
Range (%)
87-100
87-99
86-101
Average (%)
94
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.99
9.47
1.0
3.02
9.99
Standard deviation
0.06
0.13
0.28
0.06
0.12
0.35
C V (%)
6.25
4.35
2.96
6.0
3.97
3.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sdLDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sdLDL in samples. No significant cross-reactivity or interference between Human sdLDL and analogues was observed.
