Human SDF-4 (Stromal Cell Derived Factor 4) ELISA Kit
The Human SDF-4 (Stromal Cell-Derived Factor 4) ELISA Kit is a powerful tool for accurately measuring SDF-4 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and consistent results, making it ideal for a variety of research purposes.SDF-4, also known as CXCL4, is a chemokine protein that plays a critical role in immune responses, inflammation, and wound healing. Its dysregulation has been linked to various diseases, including cancer, autoimmune disorders, and cardiovascular diseases, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
By using the Human SDF-4 ELISA Kit, researchers can gain valuable insights into the role of SDF-4 in health and disease, paving the way for the development of novel treatment strategies and diagnostic tools. Trust in the accuracy and reliability of this kit to enhance your research efforts in the field of immunology and beyond.
Product Name:
Human SDF-4 (Stromal Cell Derived Factor 4) ELISA Kit
SKU:
HUES02497
Target:
Human SDF-4 (Stromal Cell Derived Factor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDF-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDF-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDF-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDF-4. You can calculate the concentration of Human SDF-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
90-107
86-100
Average (%)
90
97
93
1:4
Range (%)
87-102
83-94
84-95
Average (%)
93
89
89
1:8
Range (%)
87-98
81-93
87-100
Average (%)
93
88
92
1:16
Range (%)
87-100
85-97
88-102
Average (%)
94
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
94
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.01
228.75
818.96
109.06
210.34
780.44
Standard deviation
5.34
12.63
44.8
5.66
10.66
26.85
C V (%)
5.04
5.52
5.47
5.19
5.07
3.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SDF-4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SDF-4 in samples. No significant cross-reactivity or interference between Human SDF-4 and analogues was observed.
