The Human SDC1 (Syndecan-1) ELISA Kit is a powerful tool for the accurate measurement of Syndecan-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is specifically designed with high sensitivity and specificity to ensure precise and consistent results, making it an excellent choice for a variety of research applications.Syndecan-1 is a cell surface proteoglycan known to play a crucial role in cell signaling, cell adhesion, and cell migration. Dysregulation of Syndecan-1 expression has been linked to various diseases, including cancer, inflammation, and fibrosis.
Therefore, accurate measurement of Syndecan-1 levels can provide valuable insights into disease progression and potential therapeutic interventions.Overall, the Human SDC1 (Syndecan-1) ELISA Kit offers researchers a reliable and efficient tool for studying Syndecan-1 biology and its implications in various diseases. Its high sensitivity and specificity make it an indispensable asset for any laboratory conducting research in this area.
Product Name:
Human SDC1 (Syndecan 1) ELISA Kit
SKU:
HUES02354
Target:
Human SDC1 (Syndecan 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDC1. You can calculate the concentration of Human SDC1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
99-116
86-100
Average (%)
93
106
93
1:4
Range (%)
93-106
82-94
86-99
Average (%)
99
88
92
1:8
Range (%)
93-105
82-92
83-96
Average (%)
99
86
88
1:16
Range (%)
90-104
83-97
88-101
Average (%)
96
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.58
4.22
0.51
1.48
4.19
Standard deviation
0.03
0.09
0.2
0.03
0.07
0.22
C V (%)
5.56
5.7
4.74
5.88
4.73
5.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SDC1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SDC1 in samples. No significant cross-reactivity or interference between Human SDC1 and analogues was observed.
