Human SCFR/c-Kit (Stem Cell Factor Receptor) CLIA Kit
The Human SCFR (c-Kit) STEM CELL FACTOR RECEPTOR CLIA Kit is designed for accurate detection of stem cell factor receptor levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.The stem cell factor receptor (c-Kit) plays a crucial role in the regulation of stem cell differentiation and proliferation. It is involved in various physiological processes, including hematopoiesis, gametogenesis, and melanogenesis.
Dysregulation of c-Kit signaling has been implicated in various diseases, such as cancer and hematological disorders.By accurately measuring the levels of stem cell factor receptor in biological samples, researchers can gain insights into the role of c-Kit in disease pathogenesis and identify potential therapeutic targets. The Human SCFR (c-Kit) STEM CELL FACTOR RECEPTOR CLIA Kit provides a valuable tool for studying c-Kit signaling and developing novel interventions for related conditions.
Product Name:
Human SCFR/c-Kit (Stem Cell Factor Receptor) CLIA Kit
SKU:
HUES00752
Target:
Human SCFR/c-Kit (Stem Cell Factor Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SCFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SCFR. You can calculate the concentration of Human SCFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
96-111
96-111
Average (%)
101
101
101
1:4
Range (%)
101-118
101-113
90-102
Average (%)
109
106
95
1:8
Range (%)
98-114
98-111
87-102
Average (%)
106
104
94
1:16
Range (%)
95-111
88-98
98-109
Average (%)
102
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.05
4.6
0.53
1.05
4.43
Standard deviation
0.05
0.07
0.37
0.06
0.12
0.37
C V (%)
10.0
6.67
8.04
11.32
11.43
8.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SCFR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCFR in samples. No significant cross-reactivity or interference between Human SCFR and analogues was observed.
