Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit
The Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is a highly reliable and accurate tool for the quantitative measurement of sCD40L levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.sCD40L is a key protein involved in immune response regulation and inflammation, playing a critical role in various diseases including autoimmune disorders, cardiovascular diseases, and infectious conditions.
By accurately measuring sCD40L levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.The Human sCD40L ELISA Kit from Assay Genie provides a convenient and efficient solution for studying the role of sCD40L in health and disease. With easy-to-follow protocols and high-quality components, this kit is an essential tool for any laboratory conducting research in immunology, inflammation, and related fields.
Product Name:
Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit
SKU:
HUES01322
Target:
Human sCD40L (Soluble Cluster of Differentiation 40 Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sCD40L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sCD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sCD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sCD40L. You can calculate the concentration of Human sCD40L in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
99-115
90-103
Average (%)
99
105
96
1:4
Range (%)
90-106
82-95
82-93
Average (%)
97
88
88
1:8
Range (%)
91-104
84-95
88-99
Average (%)
98
90
94
1:16
Range (%)
92-106
81-94
88-102
Average (%)
99
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
186.51
501.84
1521.09
168.81
468.39
1530.0
Standard deviation
9.53
22.28
57.8
10.21
26.18
60.59
C V (%)
5.11
4.44
3.8
6.05
5.59
3.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sCD40L concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sCD40L in samples. No significant cross-reactivity or interference between Human sCD40L and analogues was observed.
