Human SCCA2 (Squamous Cell Carcinoma Antigen 2) ELISA Kit
The Human SCCA2 (Squamous Cell Carcinoma Antigen 2) ELISA Kit is a cutting-edge tool for precise measurement of SCCA2 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers consistent and accurate results, making it the perfect choice for various research applications.SCCA2, also known as Squamous Cell Carcinoma Antigen 2, is a vital biomarker associated with squamous cell carcinoma, a type of skin cancer.
Monitoring SCCA2 levels can provide valuable insights into disease progression and treatment response, ultimately guiding clinicians in making informed decisions.This advanced ELISA kit is a valuable resource for researchers and healthcare professionals studying squamous cell carcinoma and its associated biomarkers. It offers a reliable and efficient method for quantifying SCCA2 levels, opening new avenues for exploring potential therapies and improving patient outcomes.
Product Name:
Human SCCA2 (Squamous Cell Carcinoma Antigen 2) ELISA Kit
SKU:
HUES02711
Target:
Human SCCA2 (Squamous Cell Carcinoma Antigen 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCCA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SCCA2. You can calculate the concentration of Human SCCA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
98-111
97-114
Average (%)
97
104
104
1:4
Range (%)
91-105
86-98
87-102
Average (%)
97
91
94
1:8
Range (%)
88-103
80-93
87-102
Average (%)
95
85
93
1:16
Range (%)
91-101
81-94
85-101
Average (%)
96
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.49
3.7
0.47
1.45
3.73
Standard deviation
0.03
0.06
0.19
0.03
0.06
0.16
C V (%)
5.88
4.03
5.14
6.38
4.14
4.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SCCA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCCA2 in samples. No significant cross-reactivity or interference between Human SCCA2 and analogues was observed.
