Human SCCA1 (Squamous Cell Carcinoma Antigen 1) ELISA Kit
The Human SCCA1 (Squamous Cell Carcinoma Antigen 1) ELISA Kit is specifically designed for the accurate measurement of SCCA1 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SCCA1 is a protein biomarker commonly associated with squamous cell carcinoma, a type of skin cancer. Elevated levels of SCCA1 have been linked to the advancement and prognosis of this disease, making it a valuable marker for monitoring cancer progression and treatment efficacy.
With the Human SCCA1 ELISA Kit, researchers can confidently assess SCCA1 levels in biological samples, aiding in the understanding of squamous cell carcinoma and potentially leading to the development of targeted therapies for this aggressive form of cancer.
Product Name:
Human SCCA1 (Squamous Cell Carcinoma Antigen 1) ELISA Kit
SKU:
HUES02710
Target:
Human SCCA1 (Squamous Cell Carcinoma Antigen 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCCA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SCCA1. You can calculate the concentration of Human SCCA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
86-99
100-112
Average (%)
93
92
106
1:4
Range (%)
90-101
81-94
86-99
Average (%)
95
88
93
1:8
Range (%)
89-102
81-94
86-98
Average (%)
94
87
93
1:16
Range (%)
89-102
83-97
88-100
Average (%)
96
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
253.07
639.93
1853.36
241.86
638.79
1798.62
Standard deviation
14.86
36.41
94.52
12.48
34.3
59.17
C V (%)
5.87
5.69
5.1
5.16
5.37
3.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SCCA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCCA1 in samples. No significant cross-reactivity or interference between Human SCCA1 and analogues was observed.
