Human SAE1 Recombinant Protein (RPPB2261)
- SKU:
- RPPB2261
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- SAE1
- Synonyms:
- AOS1
- HSPC140
- SUA1
- UBLE1A
- Source:
- Escherichia Coli
- Uniprot:
- Q9UBE0
Description
| Product Name: | Human SAE1 Recombinant Protein |
| Product Code: | RPPB2261 |
| Size: | 10µg |
| Species: | Human |
| Target: | SAE1 |
| Synonyms: | AOS1, HSPC140, SUA1, UBLE1A, SAE1, SUMO1 Activating Enzyme Subunit 1, FLJ3091. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | SAE1 Human solution containing 20mM Tris pH-8, 1mM DTT & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK |
SAE1 is part of the ubiquitin-activating E1 family of proteins and participates in the significant first step of the UBL1 conjugation pathway. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteasome. SAE1 acts as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 is a dimeric enzyme that takes part as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. SAE1 regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
SAE1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 378 amino acids (1-346 a.a.) and having a molecular mass of 42.2 kDa. The SAE1 is fused to 32 amino acid T7-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:ATP-dependent protein binding; enzyme activator activity; protein binding; protein C-terminus binding; protein heterodimerization activity; SUMO activating enzyme activity; ubiquitin activating enzyme activity Biological Process: protein sumoylation; protein ubiquitination; regulation of mitotic cell cycle |
| NCBI Summary: | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
| UniProt Code: | Q9UBE0 |
| NCBI GenInfo Identifier: | 42559897 |
| NCBI Gene ID: | 10055 |
| NCBI Accession: | Q9UBE0.1 |
| UniProt Secondary Accession: | Q9UBE0,O95717, Q9P020, B2RDP5, B3KMQ2, F5GXX7, G3XAK6 |
| UniProt Related Accession: | Q9UBE0 |
| Molecular Weight: | 33,063 Da |
| NCBI Full Name: | SUMO-activating enzyme subunit 1 |
| NCBI Synonym Full Names: | SUMO1 activating enzyme subunit 1 |
| NCBI Official Symbol: | SAE1 |
| NCBI Official Synonym Symbols: | AOS1; SUA1; UBLE1A; HSPC140 |
| NCBI Protein Information: | SUMO-activating enzyme subunit 1 |
| UniProt Protein Name: | SUMO-activating enzyme subunit 1 |
| UniProt Synonym Protein Names: | Ubiquitin-like 1-activating enzyme E1A |
| Protein Family: | SUMO-activating enzyme |
| UniProt Gene Name: | SAE1 |
| UniProt Entry Name: | SAE1_HUMAN |
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