Human S100B (S100 Calcium Binding Protein B) ELISA Kit
The Human S100B (S100 Calcium Binding Protein B) ELISA Kit is a highly reliable tool for the precise measurement of S100B levels in human samples, including serum, plasma, and cell culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.S100B is a vital protein that acts as a calcium-binding protein in the body, playing a crucial role in various physiological processes.
It is known to be involved in conditions such as brain injuries, neurodegenerative diseases, and psychiatric disorders, making it an indispensable biomarker for investigating these conditions and potential therapeutic interventions.Overall, the Human S100B ELISA Kit offers researchers a valuable tool for studying the role of S100B in human health and disease, providing reliable data for further understanding its impact on various pathological processes.
Product Name:
Human S100B (S100 Calcium Binding Protein B) ELISA Kit
SKU:
HUES02353
Target:
Human S100B (S100 Calcium Binding Protein B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
88-98
89-101
Average (%)
96
93
95
1:4
Range (%)
98-115
85-97
92-104
Average (%)
106
90
98
1:8
Range (%)
99-112
87-101
94-110
Average (%)
104
93
101
1:16
Range (%)
98-112
81-95
94-106
Average (%)
104
87
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.4
168.3
729.1
107.5
175.9
681.0
Standard deviation
7.1
7.7
25.5
5.8
8.3
32.0
C V (%)
6.8
4.58
3.5
5.4
4.72
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human S100B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100B in samples. No significant cross-reactivity or interference between Human S100B and analogues was observed.
