Human RUNX2 (Runt-Related Transcription Factor 2) ELISA Kit
The Human RUNX2 (Runt-related transcription factor 2) ELISA Kit is specifically designed for the accurate measurement of RUNX2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for various research applications. RUNX2 is a critical transcription factor involved in the regulation of bone formation and development. It plays a key role in skeletal development, bone remodeling, and maintaining bone homeostasis. Dysregulation of RUNX2 has been linked to various skeletal disorders, including osteoporosis and osteosarcoma.
Therefore, accurate measurement of RUNX2 levels is essential for studying these conditions and potential therapeutic interventions. The Human RUNX2 ELISA kit provides researchers with a valuable tool for studying the role of RUNX2 in bone biology and related diseases. With its high performance and accuracy, this kit is an ideal choice for researchers looking to advance their understanding of skeletal disorders and develop new treatment strategies.
Product Name:
Human RUNX2 (Runt-Related Transcription Factor 2) ELISA Kit
SKU:
HUES02049
Target:
Human RUNX2 (Runt-Related Transcription Factor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RUNX2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RUNX2. You can calculate the concentration of Human RUNX2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
99-115
84-97
Average (%)
103
105
91
1:4
Range (%)
95-108
79-90
86-99
Average (%)
100
85
91
1:8
Range (%)
89-102
87-99
89-102
Average (%)
96
92
94
1:16
Range (%)
87-103
84-94
82-97
Average (%)
94
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
93-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.52
6.92
1.09
2.5
6.44
Standard deviation
0.06
0.14
0.32
0.07
0.12
0.26
C V (%)
5.77
5.56
4.62
6.42
4.8
4.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RUNX2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RUNX2 in samples. No significant cross-reactivity or interference between Human RUNX2 and analogues was observed.
